Journal: Biomolecules

Article Title: The Biological Effect of Enriching the Plasma Content in Platelet-Rich Plasma: An In Vitro Study

doi: 10.3390/biom14101328

Figure Lengend Snippet: Cell proliferation analysis. Cells from two phenotypes were treated with supernatants (Snts) derived from six PRP-derived formulations. The code of these supernatants consisted of two numbers referring to the plasma and platelet contents, respectively, compared to the peripheral blood. A double statistical analysis was performed to determine the effect of these supernatants on the proliferation rate induced by the specific composition of both plasma and platelet-derived factors ( A ), and, in addition, that induced by the plasma- or platelet-derived factors considered separately ( B ). The results are expressed as a percentage of the proliferation achieved by the cells maintained with the routine culture medium specific for each cell phenotype (the positive control). HDFs: human dermal fibroblasts; and HCE-1: human corneal epithelial cell. $Statistically significant differences with respect to the three times plasmatic factor group ( p ≤ 0.05 ) ( n = 5 ).

Article Snippet: The HDFs were cultured with Fibroblast Basal Medium (FM) supplemented with Fibroblast Growth Supplement (FGS), 2% FBS, and penicillin–streptomycin (all reagents provided by ScienCell Research Laboratories).

Techniques: Derivative Assay, Clinical Proteomics, Positive Control

Journal: Biomolecules

Article Title: The Biological Effect of Enriching the Plasma Content in Platelet-Rich Plasma: An In Vitro Study

doi: 10.3390/biom14101328

Figure Lengend Snippet: Cell chemotaxis analysis. Cells from two phenotypes were treated with supernatants (Snts) derived from six PRP-derived formulations. The code of these PRPs consisted of two numbers referring to the plasma and platelet contents, respectively, compared to the peripheral blood. A double statistical analysis was performed to determine the effect of these supernatants on the migration rate induced by the specific composition of both plasma- and platelet-derived factors ( A ) and, in addition, that induced by plasma or platelet-derived factors considered individually ( B ). The results are expressed as a percentage of the proliferation achieved by the cells maintained with the routine culture medium specific for each cell phenotype (the positive control). HDFs: human dermal fibroblasts; and HCE-1: human corneal epithelial cell ( n = 5 ).

Article Snippet: The HDFs were cultured with Fibroblast Basal Medium (FM) supplemented with Fibroblast Growth Supplement (FGS), 2% FBS, and penicillin–streptomycin (all reagents provided by ScienCell Research Laboratories).

Techniques: Chemotaxis Assay, Derivative Assay, Clinical Proteomics, Migration, Positive Control