Journal: Life (Basel, Switzerland)

Article Title: Eugenol Induces Apoptosis in Tongue Squamous Carcinoma Cells by Mediating the Expression of Bcl-2 Family.

doi: 10.3390/life13010022

Figure Lengend Snippet: Figure 5. Relative fold change expression of mRNA of proapoptotic (Bax and Bad) and anti-apoptotic (Bcl-2) markers in human gingival fibroblast (HGF) (a) and in tongue squamous carcinoma cells (SCC-4) (b) 72 h after exposure to Eug 0.5 mM. mRNA expression levels normalized to 18 S expression. Mean values ± SD of three independent experiments are presented. One-way ANOVA with Dunnett’s multiple comparisons post hoc test was used to identify the statistical differences (* p < 0.05 and ** p < 0.01).

Article Snippet: Fibroblast Basal Medium (ATCC® PCS-201-030TM), Fibroblast Growth Kit-Low Serum (ATCC® PCS-201-041TM), and DMEM: F-12 Medium (ATCC® 30-2006TM) were purchased from ATCC (American Type Culture Collection, Lomianki, Poland).

Techniques: Expressing

Journal: Scientific Reports

Article Title: Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions

doi: 10.1038/s41598-021-91865-1

Figure Lengend Snippet: (a) Primary fibroblasts following suspension in Poiseuille flows of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$Re=$$\end{document} R e = 5.48 for 24 hours, stained with trypan blue. (b) The viability of primary intestinal fibroblasts in circulation, assessed using MTT assay. Statistical analysis was conducted using two-way ANOVA. Results were normalised to static cells in suspension (or Re of 0). **** \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$p<$$\end{document} p < 0.0001, *** \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$p<$$\end{document} p < 0.001, ** \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$p<$$\end{document} p < 0.01, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$n=$$\end{document} n = 3 replicates, vertical bars represent the s.e.m.

Article Snippet: Intestinal fibroblasts were maintained in fibroblast basal medium (PELOBiotech, Munich, Germany), supplemented with 10% FBS (PELOBiotech, Munich, Germany), 1% antibiotic-antimycotic solution (PELOBiotech, Munich, Germany), 1% L-glutamine (PELOBiotech, Munich, Germany), 0.1% hydrocortisone (PELOBiotech, Munich, Germany) and 0.1% fibroblast growth factor (FGF), (PELOBiotech, Munich, Germany).

Techniques: Suspension, Staining, MTT Assay

Journal: Scientific Reports

Article Title: Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions

doi: 10.1038/s41598-021-91865-1

Figure Lengend Snippet: (a) The viabilities of MCF-7 and MDA-MB-231 breast cancer cells , primary T-cells, primary porcine intestinal fibroblasts and SH-SY5Y neuroblastoma cells in circulation with fitted viability lines. All viabilities are presented as a percentage of the control viability. (b) A comparative analysis of the slopes of viability. Statistical analysis was conducted using linear regression analysis. Vertical bars represent the s.e.m. (c) A comparative analysis of the elevations of viability. Statistical analysis was conducted using linear regression analysis. **** \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$p<$$\end{document} p < 0.0001, *** \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$p<$$\end{document} p < 0.001, vertical bars represent the s.e.m.

Article Snippet: Intestinal fibroblasts were maintained in fibroblast basal medium (PELOBiotech, Munich, Germany), supplemented with 10% FBS (PELOBiotech, Munich, Germany), 1% antibiotic-antimycotic solution (PELOBiotech, Munich, Germany), 1% L-glutamine (PELOBiotech, Munich, Germany), 0.1% hydrocortisone (PELOBiotech, Munich, Germany) and 0.1% fibroblast growth factor (FGF), (PELOBiotech, Munich, Germany).

Techniques: Control